Hepcidin mRNA levels in mouse liver respond to inhibition of erythropoiesis

Title:

Hepcidin mRNA levels in mouse liver respond to inhibition of erythropoiesis

Creator:

Vokurka, M., Krijt, J. , Šulc, K. , and Nečas, E.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:764e5154-66d2-4e92-b8f7-10e450aea623
uuid:764e5154-66d2-4e92-b8f7-10e450aea623

Subject:

Iron, Hepcidin, Liver, Erythropoiesis, and Gene expression

Type:

article, model:article, and TEXT

Description:

Hepcidin, a key regulator of iron metabolism, decreases intestinal absorption of iron and its release from macrophages. Iron, anemia, hypoxia, and inflammation were reported to influence hepcidin expression. To investigate regulation of the expression of hepcidin and other iron-related genes, we manipulated erythropoietic activity in mice. Erythropoiesis was inhibited by irradiation or posttransfusion polycythemia and stimulated by phenylhydrazine administration and erythropoietin. Gene expression of hepcidin and other iron-related genes (hemojuvelin, DMT1, ferroportin, transferrin receptors, ferritin) in the liver was measured by the real-time polymerase chain reaction. Hepcidin expression increased despite severe anemia when hematopoiesis was inhibited by irradiation. Suppression of erythropoiesis by posttransfusion polycythemia or irradiation also increased hepcidin mRNA levels. Compensated hemolysis induced by repeated phenylhydrazine administration did not change hepcidin expression. The decrease caused by exogenous erythropoeitin was blocked by postirradi ation bone marrow suppression. The hemolysis and anemia decrease hepcidin expression only when erythropoiesis is functional; on the other hand, if erythropoiesis is blocked, even severe anemia does not lead to a decrease of hepcidin expression, which is indeed increased. We propose that hepcidin is exclusively sensitive to iron utilization for erythropoiesis and hepatocyte iron balance, and these changes are not sensed by other genes involved in the control of iron metabolism in the liver.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2006 Volume:55 | Number:6

Harvested from:

CDK

Metadata only:

false