Sensitivity of Zebrafish (Danio rerio) embryos to hospital effluent compared to Daphnia magna and Aliivibrio fischeri

Title:

Sensitivity of Zebrafish (Danio rerio) embryos to hospital effluent compared to Daphnia magna and Aliivibrio fischeri

Creator:

Wittlerová, Martina, Jírová, Gabriela, Vlková, Alena, Kejlová, Kristina, Malý, Marek, Heinonen, Tuula, Wittlingerová, Zdeňka, and Zimová, Magdaléna

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:77955e25-1e66-4eed-9def-98bedee7255d
uuid:77955e25-1e66-4eed-9def-98bedee7255d
doi:10.33549/physiolres.934616

Subject:

Fish Embryo Toxicity (FET) test, hospital wastewater, acute toxicity, aquatic organisms, and sublethal endpoints

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

The Fish Embryo Acute Toxicity (FET) Test was adopted by the Organisation for Economic Co-operation and Development as OECD TG 236 in 2013. The test has been designed to determine acute toxicity of chemicals on embryonic stages of fish and proposed as an alternative method to the Fish Acute Toxicity Test performed according to OECD TG 203. In recent years fish embryos were used not only in the assessment of toxicity of chemicals but also for environmental and wastewater samples. In our study we investigated the acute toxicity of treated wastewater from seven hospitals in the Czech Republic. Our main purpose was to compare the suitability and sensitivity of zebrafish embryos with the sensitivity of two other aquatic organisms commonly used for wastewater testing – Daphnia magna and Aliivibrio fischeri. For the aim of this study, in addition to the lethal endpoints of the FET test, sublethal effects such as delayed heartbeat, lack of blood circulation, pericardial and yolk sac edema, spinal curvature and pigmentation failures were evaluated. The comparison of three species demonstrated that the sensitivity of zebrafish embryos is comparable or in some cases higher than the sensitivity of D. magna and A. fischeri. The inclusion of sublethal endpoints caused statistically significant increase of the FET test efficiency in the range of 1-12 %. Based on our results, the FET test, especially with the addition of sublethal effects evaluation, can be considered as a sufficiently sensitive and useful additional tool for ecotoxicity testing of the acute toxicity potential of hospital effluents.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

S681-S691

Source:

Physiological research | 2020 Volume:69 | Number:Supplement 4

Harvested from:

CDK

Metadata only:

false