Monoamine oxidase and semicarbazide-sensitive amine oxidase kinetic analysis in mesenteric arteries of patients with type 2 diabetes

Title:

Monoamine oxidase and semicarbazide-sensitive amine oxidase kinetic analysis in mesenteric arteries of patients with type 2 diabetes

Creator:

Nunes, S. F., Figueiredo, I. V., Pereira, J. S., Lemos, E. T. de, Reis, F., Teixeira, F., and Caramona, M. M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:77d21a44-235c-4c39-8e0f-60ea41356907
uuid:77d21a44-235c-4c39-8e0f-60ea41356907

Subject:

Fyziologie člověka a srovnávací fyziologie, diabetes mellitus, human mesenteric arteries, monoamine oxidase, semicarbazide-sensitive amine oxidase, type 2 diabetes, noradrenaline content, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Monoamine oxidase (MAO, type A and B) and semicarbazide-sensitive amine oxidase (SSAO) metabolize biogenic amines, however, the impact of these enzymes in arteries from patients with type 2 diabetes remains poorly understood. We investigated the kinetic parameters of the enzymes to establish putative correlations with noradrenaline (NA) content and patient age in human mesenteric arteries from type 2 diabetic patients. The kinetic parameters were evaluated by radiochemical assay and NA content by high-performance liquid chromatography (HPLC). The activity of MAO-A and SSAO in type 2 diabetic vascular tissues was significantly lower compared to the activity obtained in non-diabetic tissues. In the correlation between MAO-A (Km) and NA content, we found a positive correlation for both the diabetic and non-diabetic group, but no correlation was established for patient age. In both groups, MAO-B (Vmax) showed a negative correlation with age. The results show that MAO-A and SSAO activities and NA content of type 2 diabetic tissues are lower compared to the non-diabetic tissues, while MAO-B activity remained unchanged. These remarks suggest that MAO-A and SSAO may play an import ant role in vascular tissue as well as in the vascular pathophysiology of type 2 diabetes., S. F. Nunes ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:2

Harvested from:

CDK

Metadata only:

false