Quality of newly formed cartilaginous tissue in defects of acticular surface after transplantation of mesenchymal stem cells in a composite scaffold based on collagen I with chitosan micro- and nanofibres

Title:

Quality of newly formed cartilaginous tissue in defects of acticular surface after transplantation of mesenchymal stem cells in a composite scaffold based on collagen I with chitosan micro- and nanofibres

Creator:

Alois Nečas, Ladislav Plánka, Robert Srnec, Michal Crha, Jana Juhásová, Jiří Klíma, David Starý, Leoš Křen, Evžen Amler, Vojtová, L., Josef Jančář, and Petr Gál

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:790d26c9-5295-44a2-9eef-f06ab91fb756
uuid:790d26c9-5295-44a2-9eef-f06ab91fb756

Subject:

Fyziologie člověka a srovnávací fyziologie, kloubní chrupavka, articular cartilage, bone marrow mesenchymal stem cells, allogeneic, micropicking, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

The aim of this study was to evaluate macroscopically, histologically and immunohistochemically the quality of newly formed tissue in iatrogenic defects of articular cartilage of the femur condyle in miniature pigs treated with the clinically used method of microfractures in comparison with the transplantation of a combination of a composite scaffold with allogeneic mesenchymal stem cells (MSCs) or the composite scaffold alone. The newly formed cartilaginous tissue filling the defects of articular cartilage after transplantation of the scaffold with MSCs (Group A) had in 60 % of cases a macroscopically smooth surface. In all lesions after the transplantation of the scaffold alone (Group B) or after the method of microfractures (Group C), erosions/fissures or osteophytes were found on the surface. The results of histological and immunohistochemical examination using the modified scoring system according to O’Driscoll were as follows: 14.7±3.82 points after transplantations of the scaffold with MSCs (Group A); 5.3±2.88 points after transplantations of the scaffold alone (Group B); and 5.2±0.64 points after treatment with microfractures (Group C). The O’Driscoll score in animals of Group A was significantly higher than in animals of Group B or Group C (p<0.0005 both). No significant difference was found in the O’Driscoll score between Groups B and C. The treatment of iatrogenic lesions of the articular cartilage surface on the condyles of femur in miniature pigs using transplantation of MSCs in the composite scaffold led to the filling of defects by a tissue of the appearance of hyaline cartilage. Lesions treated by implantation of the scaffold alone or by the method of microfractures were filled with fibrous cartilage with worse macroscopic, histological and immunohistochemial indicators., A. Nečas ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:4

Harvested from:

CDK

Metadata only:

false