Autocrine effects of visfatin on hepatocyte sensitivity to insulin action

Title:

Autocrine effects of visfatin on hepatocyte sensitivity to insulin action

Creator:

Vojtěch Škop, Kontrová, K., Václav Zídek, Michal Pravenec, Ludmila Kazdová, Mikulík, K., Jiří Sajdok, and Jarmila Zídková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:79ab2823-afd1-4c51-aa78-d088b145fc82
uuid:79ab2823-afd1-4c51-aa78-d088b145fc82

Subject:

Fyziologie člověka a srovnávací fyziologie, inzulinová rezistence, RNA interference, insulin resistance, visfatin, fao hepatocytes, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Visfatin was originally described as an adipokine with insulin mimetic effects. Recently, it was found that visfatin is identical with the Nampt (nicotinamide phosphoribosyltransferase) gene that codes for an intra- and extracellular NAD biosynthetic enzyme and is predominantly expressed outside the adipose tissue. In the current study, we found strong protein and mRNA expression of visfatin in rat heart, liver, kidney, and muscle, while the expression of visfatin in visceral fat was significantly lower and undetectable in subcutaneous fat. The insulin-mimetic effects of visfatin (extracellular form of Nampt or eNampt) are controversial and even less is known about autocrine effects of visfatin (intracellular form of Nampt or iNampt). Since liver plays a major role in glucose metabolism, we studied visfatin effects on insulin-stimulated cellular glucose uptake in Fao rat hepatocytes using RNA interference (RNAi). RNAi-mediated downregulation of visfatin expression in Fao cells was associated with significantly reduced NAD biosynthesis (0.3±0.01 vs. 0.5±0.01 mmol/h/g, P<0.05) and with significantly decreased incremental glucose uptake after stimulation with insulin when compared to controls with normal expression of visfatin (0.6±0.2 vs. 2.2±0.5 nnmol/g/2 h, P=0.02). These results provide evidence that visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis., V. Škop ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:4

Harvested from:

CDK

Metadata only:

false