Different doxorubicin formulations affect plasma 4-hydroxy-2-nonenal and gene expression of aldehyde dehydrogenase 3A1 and thioredoxin reductase 2 in rat

Title:

Different doxorubicin formulations affect plasma 4-hydroxy-2-nonenal and gene expression of aldehyde dehydrogenase 3A1 and thioredoxin reductase 2 in rat

Creator:

Hlaváčová, M., Jaromír Gumulec, Tibor Stračina, Michaela Fojtů, Martina Raudenská, Michal Masařík, Nováková, M., and Hana Paulová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:79fb6634-1532-4fda-bc27-77fa10993754
uuid:79fb6634-1532-4fda-bc27-77fa10993754
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, oxidační stres, oxidative stress, doxorubicin, drug nanotransporters, 4-hydrosy-2-nonenal, thioredoxin reductase 2, aldehyde dehydrogenase 3A1, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Increased oxidative stress is indisputably an important mechanism of doxorubicin side effects, especially its cardiotoxicity. To prevent impairment of non-tumorous tissue and to improve the specificity in targeting the tumor tissue, new drug nanotransporters are developed. In many cases preclinical therapeutic advantage has been shown when compared with the administration of conventional drug solution. Three forms of doxorubicin - conventional (DOX), encapsulated in liposomes (lipoDOX) and in apoferritin (apoDOX) were applied to Wistar rats. After 24 h exposition, the plasma level of 4-hydroxy-2- nonenal (4-HNE) as a marker of lipoperoxidation and tissue gene expression of thioredoxin reductase 2 (TXNRD2) and aldehyde dehydrogenase 3A1 (ALDH3A1) as an important part of antioxidative system were determined. Only conventional DOX significantly increases the level of 4-HNE; encapsulated forms on the other hand show significant decrease in plasma levels of 4-HNE in comparison with DOX. They also cause significant decrease in gene expression of ALDH3A1 and TXNRD2 in liver as a main detoxification organ, and a mild influence on the expression of these enzymes in left heart ventricle as a potential target of toxicity. Thus, 4-HNE seems to be a good potential biomarker of oxidative stress induced by various forms of doxorubicin., M. Hlaváčová, J. Gumulec, T. Stračina, M. Fojtů, M. Raudenská, M. Masařík, M. Nováková, H. Paulová., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:Suppl 5

Harvested from:

CDK

Metadata only:

false