Effect of endothelin-1 on the excitability of rat cortical and hippocampal slices in vitro

Title:

Effect of endothelin-1 on the excitability of rat cortical and hippocampal slices in vitro

Creator:

Renata Konopková, Vilagi, I., Borbely, S., Hana Kubová, and Otáhal, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7b0a7855-6eb1-4541-9601-15b3707a0c4a
uuid:7b0a7855-6eb1-4541-9601-15b3707a0c4a

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, endothelin-1, excitability, hippocampus, somatosensory cortex, rat, epileptogenesis, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Endothelin-1 (ET-1) is a neuroactive protein produced in most brain cell types and participates in regulation of cerebral blood flow and blood pressure. In addition to its vascular effects, ET-1 affects synaptic and nonsynaptic neuronal and glial functions. Direct application of ET-1 to the hippocampus of immature rats results in cerebral ischemia, acute seizures, and epileptogenesis. Here, we investigated whether ET-1 itself modifies the excitability of hippocampal and cortical circuitry and whether acute seizures observed in vivo are due to nonvascular actions of ET-1. We used acute hippocampal and cortical slices that were preincubated with ET-1 (20 µM) for electrophysiological recordings. None of the slices preincubated with ET-1 exhibited spontaneous epileptic activity. The slope of the stimulus intensity-evoked response (input-output) curve and shape of the evoked response did not differ between ET-1-pretreated and control groups, suggesting no changes in excitability after ET-1 treatment. The threshold for eliciting an evoked response was not significantly increased in either hippocampal or cortical regions when pretreated with ET-1. Our data suggest that acute seizures after intrahippocampal application of ET-1 in rats are likely caused by ischemia rather than by a direct action of ET-1 on brain tissue., R. Konopková ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:2

Harvested from:

CDK

Metadata only:

false