Bone marrow-derived cells participate in composition of the satellite cell niche in intact and regenerating mouse skeletal muscle

Title:

Bone marrow-derived cells participate in composition of the satellite cell niche in intact and regenerating mouse skeletal muscle

Creator:

Čížková, D., Komárková, Z., Bezrouk, A., Macháčková, L., Vávrová, J., Filip, S., and Mokrý, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7c47283c-6d1d-4982-9502-4d41cc7555ee
uuid:7c47283c-6d1d-4982-9502-4d41cc7555ee

Subject:

satellite cells, bone marrow cells, satellite cell niche, transplantation, and skeletal muscle regeneration

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The cellular components of the satellite cell niche participate in the regulation of skeletal muscle regeneration. Beside myogenic cells at different developmental stages, this niche is formed by cells of the immune system, the interstitial connective tissue and the vascular ystem. Unambiguous determination of the origin of these cell types could contribute to optimization of the cell-based therapy of skeletal muscle disorders. In our work, we intravenously transplanted mouse GFP+ unseparated bone marrow cells into whole-body lethally irradiated immunocom-petent mice four weeks before cardiotoxin-induced injury of the recipients’ skeletal muscles. Seven and 28 days after the toxin injection, the injured regenerating and contralateral intact muscles were examined for identification of GFP+ bone marrow-derived cells by direct fluorescence, protein immunohistochemistry and immunogold transmission electron microscopy. In both the intact and injured muscles, GFP positivity was determined in immune cells, mainly in macrophages, and in interstitial spindle-shaped cells. Moreover, in the injured muscles, rare GFP+ endothelial cells of the blood vessels and newly formed myotubes and muscle fibres were present. Our results confirmed the ability of bone marrow-derived cells to contribute to the cellular component of the satellite cell niche in the intact and regenerating skeletal muscle. These cells originated not only from haematopoietic stem cells, but obviously also from other stem or progenitor cells residing in the bone marrow, such as multipotent mesenchymal stromal cells and endothelial progenitors. and Corresponding author: Dana Čížková

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

[155]-166

Source:

Folia biologica | 2018 Volume:64 | Number:5-6

Harvested from:

CDK

Metadata only:

false