Apoptosis of neuronal cells in the brains of postembryonic silkworms Bombyx mori (Lepidoptera: Bombycidae)

Title:

Apoptosis of neuronal cells in the brains of postembryonic silkworms Bombyx mori (Lepidoptera: Bombycidae)

Creator:

Kim, Mi Young, Kim, Kang Min, Choi, Chang Ok, Song, Hwa Young , Lee, Chang Seok, Kim , Bong Woo, Kang, Pil Don , Jeon, Soung Hoo, Cho, Byung Pil, Bae, Yeon Jae, Ko, Young-Gyo, and Lee, Bonh Hee

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7d2cc825-b7de-4a03-9215-2e668a028b11
uuid:7d2cc825-b7de-4a03-9215-2e668a028b11

Subject:

Silk moth, postembryonic life, brain, neuronal apoptosis, ecdysone, actinomycin, and caspase

Type:

article, model:article, and TEXT

Description:

The pattern and signal transduction of neuronal apoptosis in the brain of the silk moth, Bombyx mori, during postembryonic life, were characterized. Peak numbers of apoptotic neurons were detected in 4 day old 4th instar larvae, 9 day old 5th instar larvae and 4 day old pupae, indicating three waves of neuronal apoptosis during postembryonic development. Most of the apoptotic neurons were in the lateral portions of the brain. No apoptotic neurons were detected in 1 day old 1st instar larvae or in 7 day old pupae to 1 day old adults. Injection of 20-hydroxyecdysone (20E) into larvae resulted in a substantial increase in the brain in both neuronal apoptosis and cleavage of procaspases-8 and -3 into caspases-8 and -3. However, the injection of larvae with actinomycin D or cycloheximide inhibited death of pre-apoptotic neurons. Both the cleavage of procaspases-8 and -3 and death of pre-apoptotic neurons were inhibited by a general caspase inhibitor and caspase-8 and -3 inhibitors injected into larvae. These results suggest that 20E triggered the synthesis of a new protein that, in turn, induces cleavage of procaspases-8 and -3 into caspases-8 and -3. These caspases are prerequisites for neuronal apoptosis in postembryonic brains.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2009 Volume:106 | Number:3

Harvested from:

CDK

Metadata only:

false