Different secretory response of pancreatic islets and insulin secreting cell lines INS-1 and INS-1E to osmotic stimuli

Title:

Different secretory response of pancreatic islets and insulin secreting cell lines INS-1 and INS-1E to osmotic stimuli

Creator:

Orečná, M., Hafko, R., Bačová, Z., Jarmila Vargová, Dušan Chorvát, and Vladimír Štrbák

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7ee4ce98-14cc-4ffc-acf6-7d52656da303
uuid:7ee4ce98-14cc-4ffc-acf6-7d52656da303
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, endokrinologie, inzulin, nádorové buňky, pankreas, endocrinology, insulin, cancer cells, pancreas, cell volume, pancreatic islets, tumor cell line, Ca2+ and exocytosis, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Objective of this study was to characterize osmotically-induced insulin secretion in two tumor cell lines. We compared response of freshly isolated rat pancreatic islets and INS-1 and INS-1E tumor cell lines to high glucose, 30 % hypotonic medium and 20 % hypertonic medium. In Ca2+-containing medium glucose induced insulin release in all three cell types. Hypotonicity induced insulin secretion from islets and INS-1 cells but not from INS-1E cells, in which secretion was inhibited despite similar increase in cell volume in both cell types. GdCl3 (100 μmol/l) did not affect insulin response from INS-1E cells to hypotonic challenge. Hypertonic medium inhibited glucose-induced insulin secretion from islets but not from tumor cells. Noradrenaline (1 μmol/l) inhibited glucose-induced but not swelling-induced insulin secretion from INS-1 cells. Surprisingly, perifusion with Ca2+-depleted medium showed distinct secretory response of INS-1E cells to hypotonicity while that of INS-1 cells was partially inhibited. Functioning glucose-induced insulin secretion is not sufficient prerequisite for hypotonicity-induced response in INS-1E cells suggesting that swelling-induced exocytosis is not essential step in the mechanism mediating glucose-induced insulin secretion. Both cell lines are resistant to inhibitory effect of hyperosmolarity on glucose-induced insulin secretion. Response of INS-1E cells to hypotonicity is inhibited by the presence of Ca2+ in medium., M. Orečná, R. Hafko, Z. Bačová, J. Podskočová, D. Chorvát Jr., V. Štrbák., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:6

Harvested from:

CDK

Metadata only:

false