Inhibitory effect of metformin on oxidation of NADH-dependent substrates in rat liver homogenate

Title:

Inhibitory effect of metformin on oxidation of NADH-dependent substrates in rat liver homogenate

Creator:

Páleníčková, E., Monika Cahová, Zdeněk Drahota, and Martin Kalous

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:816237f6-6b0a-4b35-a97f-1955baea7ced
uuid:816237f6-6b0a-4b35-a97f-1955baea7ced

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, liver homogenate, glutamate and palmityl carnitine oxidation, metformin, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Metformin is widely used in the treatment of Type 2 diabetes, however, mechanisms of its antihyperglycemic effect were not yet fully elucidated. Complex I of mitochondrial respiration chain is considered as one of the possible targets of metformin action. In this paper, we present data indicating that the inhibitory effect of metformin can be tested also in liver homogenate. Contrary to previous findings on hepatocytes or mitochondria under our experimental conditions, lower metformin concentrations and shorter time of preincubation give significant inhibitory effects. These conditions enable to study the mechanism of the inhibitory effect of metformin in small samples of biological material (50-100 mg wet weight) and compare more experimental groups of animals because isolation of mitochonria is unnecessary., E. Páleníčková ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:5

Harvested from:

CDK

Metadata only:

false