Effect of Stobadine on Opsonized Zymosan Stimulated Generation of Reactive Oxygen Species in Human Blood Cells

Title:

Effect of Stobadine on Opsonized Zymosan Stimulated Generation of Reactive Oxygen Species in Human Blood Cells

Creator:

Pečivová, J., Mačičková, T., Číz, M., Nosál, R., and Lojek, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:84990d16-ac23-4168-85f0-be7422567f6d
uuid:84990d16-ac23-4168-85f0-be7422567f6d

Subject:

Human blood cells, Opsonized zymosan, Stobadine, and Reactive oxygen species

Type:

article, model:article, and TEXT

Description:

To predict more precisely the effect of stobadine, a pyridoindole antioxidant agent, in the whole organism, we studied its effect on opsonized zymosan-stimulated free radical generation in whole blood, on superoxide generation in the mixture of PMNL : platelets (1:50), as well as on superoxide generation and myeloperoxidase release in isolated PMNL. Without stimulation, stobadine had no effect on reactive oxygen species (ROS) generation and myeloperoxidase release. Stobadine in a concentration of 10 or 100 µmol/l significantly decreased luminol-enhanced chemiluminescence in opsonized zymosan-stimulated whole blood. In concentrations of 10 and 100 µmol/l, it reduced myeloperoxidase release from isolated neutrophils. Stobadine significantly decreased superoxide generation in isolated neutrophils in 100 µmol/l concentration. Its effect was much less pronounced in the mixture of neutrophils and platelets in the ratio close to physiological conditions (1:50). Our results suggest that stobadine might exert a beneficial effect in diseases or states where superfluous ROS generation could be deleterious.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:1

Harvested from:

CDK

Metadata only:

false