RNA interference mediated knockdown of an inhibitor of apoptosis protein induces apoptosis in Mythimna separata (Lepidoptera: Noctuidae)

Title:

RNA interference mediated knockdown of an inhibitor of apoptosis protein induces apoptosis in Mythimna separata (Lepidoptera: Noctuidae)

Creator:

Kamezaki, Masashi, Yokoi, Kakeru, and Miura, Ken

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8637f56a-8e2d-499d-a1cf-8d0f896fcdc4
uuid:8637f56a-8e2d-499d-a1cf-8d0f896fcdc4
issn:1210-5759
doi:10.14411/eje.2018.021

Subject:

motýli, můrovití, apoptóza, butterflies, Noctuidae, apoptosis, Mythimna separata, hemocytes, caspase, cDNA, RNA interference, inhibitor of apoptosis protein, TUNEL strain, 2, and 59

Type:

model:article and TEXT

Format:

print, počítač, and online zdroj

Description:

Coordinated regulation of apoptosis is critical for development, homeostasis, and immunity in larvae of Metazoa. We determined the full nucleotide sequence of an inhibitor of an apoptosis protein in a lepidopteran insect Mythimna separata (Walker) (MsIAP) and carried out functional analyses of the MsIAP. The full-length cDNA of MsIAP was 1642 bp, which encoded 379 amino acid residues with a calculated molecular mass of 41,834 Da, and two BIR domains and one RING domain revealed using amino acid sequence analysis. In addition, the sequences of these domains were similar to Drosophila IAP1 and those of some other lepidopteran insects. We carried out a functional analyses of MsIAP related to apoptosis regulation using RNA interference. The effects of MsIAP knockdown on adhering hemocytes and non-adhering hemocytes as controls were examined using Hoechst33342/propidium iodide staining, effector caspase activity and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) staining. A significantly higher number of propidium iodide and TUNEL-positive cells was recorded in adhering hemocytes from MsIAP knockdown larvae than from control larvae, but these differences were not recorded for non-adhering hemocytes. However, higher effector caspase activity was detected in both adhering and non-adhering hemocytes from MsIAP knockdown larvae compared to that in control larvae. These results indicate that the knockdown of MsIAP induces apoptosis in larval adhering hemocytes, which MsIAP negatively and non-redundantly regulate apoptosis, and that IAP function is conserved in M. separata and other insect species including Drosophila and several lepidopteran insects., Masashi Kamezaki, Kakeru Yokoi, Ken Miura., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2018 Volume:115 | Number:1

Harvested from:

CDK

Metadata only:

false