Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells

Title:

Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells

Creator:

Pechová, Jitka, Kopecký, Jan, and Salát, Jiří

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:87903855-2e22-4127-9e45-5eb0bca6c18d
uuid:87903855-2e22-4127-9e45-5eb0bca6c18d
doi:10.14411/fp.2004.046

Subject:

Ixodes ricinus, salivary gland extract, Borrelia afzelii, mouse epidermal cells, and cytokines

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Previous studies have demonstrated that both tick saliva and Borrelia burgdorferi sensu lato antigens modulate the cytokine response of the host. In this paper, the effect of salivary gland extract (SGE) from Ixodes ricinus (L., 1758) ticks on cytokine production by primary cultures of mouse epidermal cells stimulated with Borrelia afzelii Canica, Nato, du Merle, Mazie, Baranton et Postic, 1993 spirochetes was analysed. Epidermal cells were derived from C3H/HeN mice, susceptible to Lyme disease, and BALB/c mice, which are resistant. In cultures from C3H/HeN mice, SGE down regulated production of tumour necrosis factor alpha (TNF-α) and up regulated Th2 cytokine, interleukin 4 (IL-4). Cultures from BALB/c mice produced higher basal levels of monitored cytokines, but their production was affected by SGE a different way. While Th2 cytokines IL-6 and IL-10 were down regulated, the effect on TNF-α and IL-4 was ambiguous. These results indicate that the effect of tick saliva on the epidermal cells of Lyme disease-susceptible C3H/HeN mice mirrors its effect on other cells of the immune system.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

367-372

Source:

Folia parasitologica | 2004 Volume:51 | Number:4

Harvested from:

CDK

Metadata only:

false