Vascular metabolic dysfunction and lipotoxicity

Title:

Vascular metabolic dysfunction and lipotoxicity

Creator:

Mattern, H. M. and Hardin, C. D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:87f8bf66-0c12-4d2e-89ec-350fbe74b597
uuid:87f8bf66-0c12-4d2e-89ec-350fbe74b597
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, cévní, apoptóza, physiology, smooth muscles, apoptosis, hladké svaly cév, palmitát, oleát, reaktivní formy kyslíku, vascular smooth muscle, palmitate, oleate, reactive oxygen species, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The purpose of this study was to determine the role of lipotoxicity in vascular smooth muscle (VSM). C1-BODIPY 500/510 C12 used to assess the ability of VSM A7r5 cells to transport long-chain fatty acids showed that lipid transport did not appear to limit metabolism. Thin layer chromatography revealed that storage of transported fatty acid occurred primarily as mono- and diglycerides and fatty acids but not as triglycerides. We used lipid-induced apoptosis as a measure of lipotoxicity and found that 1.5 mM palmitate (6.8:1) bound to albumin resulted in a 15-fold increase in the number of apoptotic cells compared to the control at 24 hours. This apoptosis did not seem to be due to an increase in reactive oxygen species (ROS) since VSM cells incubated in palmitate showed less ROS production than cells incubated in albumin only. Similar exposure to oleate did not significantly increase the number of apoptotic cells compared to the control. Oleate actually significantly attenuated the apoptosis induced by palmitate, suggesting that unsaturated fatty acids have a protective effect on cells undergoing palmitate-induced apoptosis. These results suggest that vascular smooth muscle is vulnerable to lipotoxicity and that this lipotoxicity may play a role in the development of atherosclerosis., H. M. Mattern, C. D. Hardin., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:2

Harvested from:

CDK

Metadata only:

false