Developmentally regulated expression of surface carbohydrate residues on larval stages of the avian schistosome Trichobilharzia szidati

Title:

Developmentally regulated expression of surface carbohydrate residues on larval stages of the avian schistosome Trichobilharzia szidati

Creator:

Horák, P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:886a8819-45e7-47e0-a613-11de08b9d039
uuid:886a8819-45e7-47e0-a613-11de08b9d039
urnnbn:urn:nbn:cz:aba007-000dkf

Subject:

Carbohydrates, lectin, receptor, schistosomes, Trichobilharzia, Lymnaea stagnalis, immunity, and immune evasion

Type:

model:internalpart and TEXT

Format:

print

Description:

F.xcept other functions, surface saccharide residues on trematode larvae are supposed either to be the targets of the intermediate (molluscan) and final host immune systems, or to represent candidates for molecular mimicry. Therefore, changes in surface saccharide patterns during the development of the avian schistosome Trichobilharzia szidati were characterized. Whole parasite larval stages and their tissue sections were examined using FITC-conjugated lectins. Marked surface differences were found among larval stages (miracidia, mother sporocysts, daughter sporocysts, cercariae, schistosomula). Staining by some lectins reflected known ultrastructural changes of the outer tegument. Reaction of lectins with cercarial embryos was almost negative. In case of other developmental stages, binding of at least one member from each carbohydrate-specificity group of lectins (Man/Glc-, GIcNAc-, Gal/GalNAc- and Fuc-specific) occurred. One exception is represented by mother and daughter sporocysts which practically failed to react with Fuc-specific lectins. Besides other lectins which recognized larval surfaces, a-L-fucose-specific lectins (LTA, UEA-I) and (GlcNAcfll —>4)„-spccific WGA bound very strong to certain stages. The comparison of mature intrasporocystic cercariae with those emerged from snails brought the indication that some snail glycosylated molecules adhere to the surface of schistosome larvae or that emerged cercariae express some new carbohydrate epitopes under changed environmental conditions. The result partially supports the theory of parasite mimicry/masking strategies and immune evasion in the host.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

255-265 and 17-27

Source:

Folia parasitologica | 1995 Volume:42 | Number:4

Harvested from:

CDK

Metadata only:

false