What is the source of free radicals causing hemolysis in stored blood?

Title:

What is the source of free radicals causing hemolysis in stored blood?

Creator:

Jaroslav Racek, Růžena Herynková, Václav Holeček, Faltysová, J., and Krejčová, I.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:89aa6045-b74d-4b50-bd91-1b7d78f952bf
uuid:89aa6045-b74d-4b50-bd91-1b7d78f952bf
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, volné radikály, leukocyty, hemolýza, free radicals, leucocytes, hemolysis, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The quality of stored blood can be deteriorated by hemolysis caused by free radicals. The purpose of this study was to elucidate whether neutrophile leukocytes are the source of free radicals in stored blood as in hemodialyzed patients. Resuspensions with low (LL) or high (HL) leukocyte concentrations were prepared from samples of twenty healthy volunteers. The samples were incubated for 10 days at 4 °C and then for one day at 37 °C. Markers of hemolysis and free radical metabolism were examined before and after incubation in LL and HL samples. In spite of the difference of leukocytes counts in LL and HL resuspensions (p<0.0001), the pre-incubation values of all laboratory parameters were practically identical. In post-storage samples, superoxide dismutase and glutathione peroxidase activities did not differ in either group. Reduced glutathione in erythrocytes and extracellular antioxidant capacity were insignificantly lower in HL resuspensions, but the increase of malondialdehyde was much more pronounced in the HL samples (p<0.0001). The degree of hemolysis, expressed as the extracellular increase of potassium (p<0.001), hemoglobin (p<0.05) and lactate dehydrogenase (p<0.05), was higher in the HL samples. Our results support the hypothesis that leukocytes participate in free radical production in stored blood., J. Racek, R. Herynková, V. Holeček, J. Faltysová, I. Krejčová., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2001 Volume:50 | Number:4

Harvested from:

CDK

Metadata only:

false