Cyclosporin a modifies cytoplasmic calcium levels in isolated hepatocytes exposed to oxidative stress due to tert-butyl hydroperoxide

Title:

Cyclosporin a modifies cytoplasmic calcium levels in isolated hepatocytes exposed to oxidative stress due to tert-butyl hydroperoxide

Creator:

Eva Kmoníčková, Ludmila Kameníková, Sixtus Hynie, and Hassan Farghali

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8a570021-b673-4ac0-bf39-ea3ddd2e3825
uuid:8a570021-b673-4ac0-bf39-ea3ddd2e3825
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hypertenze, hypertension, rat hepatocytes, Ca2+i, cyclosporin A, 14, and 612

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Within the framework of our studies on hypertension in various rat strains, we have examined the effect of cyclosporin A (CsA) on intracellular calcium signaling under conditions of oxidative stress. For these preliminary experiments, we have chosen isolated hepatocytes of normotensive rats as a model system for the study of the role of intracellular calcium. We used tert-butyl hydroperoxide (t-BHP, 1 mmol.l-1) as an prooxidant agent. When compared to the controls, we found increased levels of cytosolic free calcium concentration (Ca2+i) during 120 min incubation. The preincubation of hepatocytes with CsA in the concentration of 0.5 m mol.l-1 did not change the physiological level of cytosolic calcium. However, a dual action of CsA on elevated Ca2+i was observed during oxidative injury of hepatocytes: while in the first period of incubation CsA increased Ca2+i, CsA reduced the effect of t-BHP on Ca2+i during the next period of incubation. This indicates the ability of CsA to modify oxidative stress, but further studies are necessary to explain these findings., E. Kmoníčková, L. Kameníková, S. Hynie, H. Farghali., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2000 Volume:49 | Number:4

Harvested from:

CDK

Metadata only:

false