Influence of collagen and chondroitin sulfate (CS) coatings on poly-(lactide-coglycolide) (PLGA) on MG 63 osteoblast-like cells

Title:

Influence of collagen and chondroitin sulfate (CS) coatings on poly-(lactide-coglycolide) (PLGA) on MG 63 osteoblast-like cells

Creator:

Vandrovcová, M., Douglas, T., Hauk, D., Grössner-Schreiber, B., Wiltfang, J., Lucie Bačáková, and Warnke, P. H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8bfbe1e5-79b6-4408-812d-18bee3ff6ee6
uuid:8bfbe1e5-79b6-4408-812d-18bee3ff6ee6

Subject:

Fyziologie člověka a srovnávací fyziologie, kolagen, biodegradovatelné polymery, osteoklasty, popraviště, collagen, biodegradable polymers, osteoclasts, scaffolds, glycosaminoglycan, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Poly-(lactide-co-glycolide) (PLGA) is an FDA-approved biodegradable polymer which has been widely used as a scaffold for tissue engineering applications. Collagen has been used as a coating material for bone contact materials, but relatively little interest has focused on biomimetic coating of PLGA with extracellular matrix components such as collagen and the glycosaminoglycan chondroitin sulfate (CS). In this study, PLGA films were coated with collagen type I or collagen I with CS (collagen I/CS) to investigate the effect of CS on the behaviour of the osteoblastic cell line MG 63. Collagen I/CS coatings promoted a significant increase in cell number after 3 days (in comparison to PLGA) and after 7 days (in co mparison to PLGA and collagen-coated PLGA). No influence of collagen I or collagen I/CS coatings on the spreading area after 1 day of culture was observed. However, the cells on collagen I/CS formed numerous filopodia and displayed well developed vinculin-containing focal adhesion plaques. Moreover, thes e cells contained a significantly higher concentration of osteocalcin, measured per mg of protein, than the cells on the pure collagen coating. Thus, it can be concluded that collagen I/CS coatings promote MG 63 cell proliferation, improve cell adhesion and enhance osteogenic cell differentiation., M. Vandrovcová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:5

Harvested from:

CDK

Metadata only:

false