Identification of microsatellite markers for a worldwide distributed, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidae)

Title:

Identification of microsatellite markers for a worldwide distributed, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidae)

Creator:

Jan Zima, Lebrasseur, Ophélie, Michaela Borovanská, and Janda, Milan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:900da785-5dca-498f-8725-94d7aed99c8b
uuid:900da785-5dca-498f-8725-94d7aed99c8b
issn:1210-5759
doi:10.14411/eje.2016.053

Subject:

Zoologie, blanokřídlí, Hymenoptera, Formicidae, Tapinoma melanocephalum, microsatellites, invasive species, homozygosity excess, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, počítač, and online zdroj

Description:

Tapinoma melanocephalum is a worldwide distributed, highly invasive ant species. It lives in close association with human societies and its distribution is human-mediated in large measure. The geographical origin of this ant species is unknown, but its introduction in areas previously devoided of its presence can represent a threat to the native biota, act as an agricultural pest or as a pathogen vector. To investigate the genetic structure and phylogeography of this species we identified 12 new polymorphic microsatellite markers, and in addition, we tested and selected 12 ant-universal microsatellites polymorphic in T. melanocephalum. We genotyped 30 individuals from several islands of Micronesia and Papua-New Guinea. All 24 loci exhibited strong homozygosity excess (45-100%, mean = 86%), while the number of alleles per locus reached usual values (2-18, mean = 6.5), resulting in levels of expected heterozygosity much higher than observed. Based on several robust tests, we were able to exclude artefacts such as null alleles and allelic dropout as a possible cause of the observed pattern. Homozygosity excess might be a consequence of founder effect, bottleneck and/or inbreeding. As our sample population was composed of individuals from several distinct localities, the Wahlund effect might have contributed to the increased homozygosity as well. Despite the provisionally observed deviation from the Hardy-Weinberg equilibrium, the newly developed microsatellites will provide an effective tool for future genetic investigations of population structure as well as for the phylogeographic study of T. melanocephalum., Jan Zima Jr., Ophélie Lebrasseur, Michaela Borovanská, Milan Janda., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

European Journal of Entomology | 2016 Volume:113

Harvested from:

CDK

Metadata only:

false