Increased gene expression and production of spinal cyclooxygenase 1 and 2 during experimental osteoarthritis pain

Title:

Increased gene expression and production of spinal cyclooxygenase 1 and 2 during experimental osteoarthritis pain

Creator:

Michaela Procházková, Peter Zanvit, Tomáš Doležal, Ludmila Prokešová, and Miloslav Kršiak

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9067c9de-a172-49ad-97c6-c29c36442daf
uuid:9067c9de-a172-49ad-97c6-c29c36442daf
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, bolest, artritida, cyklooxygenáza 2, mícha, pain, arthritis, cyclooxygenase 2, spinal cord, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Knowledge on the involvement of spinal COX-1 and COX-2 in pain due to osteoarthritis could be useful for better understanding of its pa thogenesis and therapy. In this study we have investigated a long-term pattern of expression and production of spinal COX-1 and COX-2 in the model of osteoarthritis induced in rats by injection of monoiodoacetate (MIA) into the knee joint. MIA injection produced thermal hyperalgesia (assessed by the plantar test) and tactile allodynia (measured with von Frey hairs). The pain measures reached maximum on the 5th day, then re mained relatively stable. The expression of spinal COX-2 mR NA reached maximum on day 5 (5.2 times; P<0.001) and remain ed increased until day 31 (4.9 times; P<0.001). Expression of spinal COX-1 mRNA increased gradually reaching maximum on the day 31 (4.5 times; P<0.001) when the relative expression of both genes was almost equal. The production of both proteins was almost similar at the beginning of the experiment. The highest production of COX-2 protein was observed on day 5 after the induction of osteoarthritis (increased 3.9 times). The levels of COX-1 protein increased gradually with maximum on day 31 (3.4 times). The present findings indicate that not only expression of COX-2 mRNA but also that of COX-1 mRNA is significantly increased in the spine during osteoarthritis pain. Thus, in contrast to inflammatory pain, the upregulation of spinal COX-1 may be important in osteoarthritis pain., M. Procházková, P. Zanvit, T. Doležal, L. Prokešová, M. Kršiak., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2009 Volume:58 | Number:3

Harvested from:

CDK

Metadata only:

false