Effects of SERCA and PMCA inhibitors on the survival of rat cochlear hair cells during ischemia in vitro

Title:

Effects of SERCA and PMCA inhibitors on the survival of rat cochlear hair cells during ischemia in vitro

Creator:

Amarjargal, N., Mazurek, B., Haupt, H., Nadežda Andrejeva, Fuchs, J., and Gross, Johann

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9596e512-8460-41d5-a6ce-7b7dc191f976
uuid:9596e512-8460-41d5-a6ce-7b7dc191f976
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, molekulární biologie, vápník, ischemie, potkan, molecular biology, calcium, ischemia, Rattus norvegicus, organ of Corti, plasma membrane calcium ATPase (PMCA), 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

An important mechanism underlying cochlear hair cell (HC) susceptibility to hypoxia/ischemia is the influx of Ca2+. Two main ATP-dependent mechanisms contribute to maintaining low Ca2+ levels: uptake of Ca2+ into intracellular stores via smooth endoplasmic reticulum calcium ATPase (SERCA) and extrusion of Ca2+ via plasma membrane calcium ATPase (PMCA). The effects of the SERCA inhibitors thapsigargin (10 nM-10 μM) and cyclopiazonic acid (CPA; 10-50 μM) and of the PMCA blockers eosin (1.5-10 μM) and o-vanadate (1-5 mM) on inner and outer hair cells (IHCs/OHCs) were examined in normoxia and ischemia using an in vitro model of the newborn rat cochlea. Exposure of the cultures to ischemia resulted in a significant loss of HCs. Thapsigargin and CPA had no effect. Eosin decreased the numbers of IHCs and OHCs by up to 25 % in normoxia and significantly aggravated the ischemia-induced damage to IHCs at 5 and 10 μM and to OHCs at 10 μM. o-Vanadate had no effect on IHC and OHC counts in normoxia, but aggravated the ischemia-induced HC loss in a dose-dependent manner. The effects of eosin and o-vanadate indicate that PMCA has an important role to play in protecting the HCs from ischemic cell death., N. Amarjargal, B. Mazurek, H. Haupt, N. Andeeva, J. Fuchs, J. Gross., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:4

Harvested from:

CDK

Metadata only:

false