Expression of osteogenic factors in FasL-deficient calvarial cells

Title:

Expression of osteogenic factors in FasL-deficient calvarial cells

Creator:

Kratochvilova, Adela, Stouracova, Sabina, Oralova, Veronika, Gruber, Reinhard, Doubek, Jaroslav, and Matalova, Eva

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:97f530ab-721e-4c18-8e0d-ad4878067333
uuid:97f530ab-721e-4c18-8e0d-ad4878067333
doi:10.33549/physiolres.934945

Subject:

FasL, osteogenesis, calvarial cells, Rankl, and Opg

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

During bone development, FasL acts not only through the traditional apoptotic mechanism regulating the amount of boneresorbing osteoclasts, but there is also growing evidence about its effect on cell differentiation. Expression of osteoblastic factors was followed in non-differentiated and differentiating primary calvarial cells obtained from FasL-deficient (gld) mice. The gld cells showed decreased expression of the key osteoblastic molecules osteocalcin (Ocn), osteopontin (Opn), and alkaline phosphatase (Alpl) in both groups. Notably, receptor activator of nuclear factor kappa-B ligand (Rankl) was unchanged in nondifferentiated gld vs. wild type (wt) cells but decreased in differentiating gld cells. Osteoprotegerin (Opg) in the gld samples was increased in both groups. Opg vs. Rankl expression levels favored Opg in the case of non-differentiated cells but Rankl in differentiating ones. These results expand information on the involvement of FasL in non-apoptotic cell pathways related to osteoblastogenesis and consequently also osteoclastogenesis and pathologies such as osteoporosis.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

117-121

Source:

Physiological research | 2023 Volume:72 | Number:1

Harvested from:

CDK

Metadata only:

false