Molecular cloning and characterization of the S6K-p70 gene in Chinese honeybees, Apis cerana cerana (Hymenoptera: Apidae)

Title:

Molecular cloning and characterization of the S6K-p70 gene in Chinese honeybees, Apis cerana cerana (Hymenoptera: Apidae)

Creator:

Cai, Yingqi, Ai, Taobo, Yu, Xiaoli, Xu, Baohua, and Guo, Xingqi

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:980bcee3-3f71-4882-9997-e51474bb0761
uuid:980bcee3-3f71-4882-9997-e51474bb0761

Subject:

Zoologie, zoologie, entomologie, Apidae, ribosomal protein S6 kinase, Apis cerana cerana, development, environmental stresses, expression pattern, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Description:

The ribosomal protein S6 kinase (S6K) plays a pivotal role in developmental processes and cell survival by participating in protein synthesis relevant signaling pathways. In the present study, an S6K gene (AccS6K-p70) was isolated and characterized from the Chinese honeybee, Apis cerana cerana (Fabricius) (Hymenoptera: Apidae), an important economic insect in the agricultural industry. The cDNA of AccS6K-p70 was 1683 bp in length and predicted to encode a protein of 467 amino acid residues. Sequence and structure analysis showed that there was a conserved catalytic domain in AccS6K-p70, whilst a phosphorylation site was found in the conserved part of the catalytic domain. Development relevant transcription factor binding sites found in the 5’-flanking region of AccS6K-p70 suggest that AccS6K-p70 might be involved in A. c. cerana development. Furthermore, quantitative PCR revealed that the expression levels of AccS6K-p70 were higher in head and thorax than in other tissues. The AccS6K-p70 was highly expressed in both larvae and adults compared with that in pupae, whilst expression of the gene was significantly down-regulated by hydrogen peroxide (H2O2) (although initially and slightly increased by it) and pyriproxyfen (a juvenile hormone analogue insecticide) stresses. These results suggest that AccS6K-p70 may play critical roles in developmental processes and cell survival in A. c. cerana, whilst both oxidative stress and pyriproxyfen may impair S6K-p70 mediated developmental processes by down-regulation of AccS6K-p70 expression., Yingqi Cai ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2013 Volume:110 | Number:1

Harvested from:

CDK

Metadata only:

false