miR-29a is a potential protective factor for fibrogenesis in gluteal muscle contracture

Title:

miR-29a is a potential protective factor for fibrogenesis in gluteal muscle contracture

Creator:

Zhou, Ri, Ren, Shiyou, Li, Canfeng, Zhang, Xintao, and Zhang, Wentao

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9ac7c4ef-cae1-4f6b-a73b-da03f17d1050
uuid:9ac7c4ef-cae1-4f6b-a73b-da03f17d1050
doi:10.33549/physiolres.934295

Subject:

gluteal muscle contracture, miR-29a, fibrosis, HSP47, and TGF-β1

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Circulating miRNAs have been proposed as the effective diagnostic biomarkers for muscular fibrosis-associated diseases. However, circulating biomarkers for early diagnosis of contracture muscles are limited in gluteal muscle contracture (GMC) patients. Here we sought to explore the abnormally expressed miRNAs in plasma and contraction bands of GMC patients. The results showed miR-29a-3p expression in plasma and contraction bands tissue was significantly reduced in GMC patients compared with normal control. Cell viability and levels of proliferation-associated protein cyclin D1 and cyclindependent-kinase 2 (CDK2) were powerfully inhibited by miR-29a mimics and enhanced by miR-29a inhibitor compared with negative control. Furthermore, miR-29a mimics effectively impeded, while miR-29a inhibitor enhanced the expression of collagen I and collagen III, followed by the secretion of transforming growth factor β1 (TGF-β1), TGF-β3 and connective tissue growth factor (CTGF) in primary human contraction bands (CB) fibroblasts. The miR-29a-3p negatively regulated the expression of TGF-β1 through binding to the 3′ UTR region of SERPINH1 (encoding heat shock protein HSP47), but had no effect on Smad2 activity. The miR-29a-3p was inversely correlated with HSP47 in contraction bands tissue from GMC patients. Collectively, miR-29a was notably depressed and regulated cell viability and fibrosis by directly targeting HSP47 in GMC, which suggest that circulating miR-29a might be a potential biomarker for early diagnosis and provides a novel therapeutic target for GMC.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

467-479

Source:

Physiological research | 2020 Volume:69 | Number:3

Harvested from:

CDK

Metadata only:

false