Flight substrates in blister beetles (Coleoptera: Meloidae) and their regulation by neuropeptides of the AKH/RPCH family

Title:

Flight substrates in blister beetles (Coleoptera: Meloidae) and their regulation by neuropeptides of the AKH/RPCH family

Creator:

Gäde, Gerd and Auerswald, Lutz

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9d186aca-c768-40cd-ae44-75971d281b52
uuid:9d186aca-c768-40cd-ae44-75971d281b52

Subject:

zoologie, entomologie, hmyz, brouci, Meloidae, Decapotoma lunata, Mylabris oculata, let, letové substráty, hemolymfa, tuky, cukry, proline, koncentrace, regulace koncentrace substrátů, neuropeptidy, AKH/RPCH neuropeptidy, 595.2/.7, and 591.1

Type:

article, bibliography, model:article, and TEXT

Format:

print, text, and regular print

Description:

We measured energy substrates in haemolymph and flight muscles of the large blister beetle Mylabris oculata at rest and after tethered, lift-generating flight. Flight of 1 min duration at an ambient temperature of 38-42°C did not effect a change in the concentration of lipids in the haemolymph, whereas a small, significant decrease in the concentrations of carbohydrates and a 3-fold larger one in the levels of proline were noted, as well as a concomitant increase in alanine. In the flight muscles, glycogen and proline concentrations were diminished slightly but significantly upon flight, whereas alanine levels were increased. Two hours of rest after a flight of 1 min completely reversed the metabolic situation in haemolymph and flight muscles to pre-flight levels. We could isolate two neuropeptides from the corpora cardiaca of M. oculata, which by retention time and mass analyses are characterised as the decapeptide Del-CC (pGlu-Leu-Asn-Phe-Ser-Pro-Asn-Trp-Gly-Asn-NH2) and the octapeptide Tem-HrTH (pGlu-Leu-Asn-PheSer-Pro-Asn-Trp-NH2) previously fully identified from the corpora cardiaca of the blister beetle, Decapotoma lunata (Gäde, 1995). Subsequently, it was unequivocally demonstrated that low doses of Del-CC and Tem-HrTH elicited increases in the concentration of proline and carbohydrates in the haemolymph of D. lunata and M. oculata, but did not change the concentration of lipids in both species. In conclusion, the two endogenous peptides are hypertrehalosaemic and hyperprolinaemic, thus very likely regulating the mobilisation of the two important flight substrates of blister beetles, namely carbohydrates and proline., Gerd Gäde, Lutz Auerswald, and Lit

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 1999 Volume:96 | Number:3

Harvested from:

CDK

Metadata only:

false