Effects of Human Soluble BAFF Synthesized in Escherichia coli on CD4+ and CD8+ T Lymphocytes as well as NK Cells in Mice

Title:

Effects of Human Soluble BAFF Synthesized in Escherichia coli on CD4+ and CD8+ T Lymphocytes as well as NK Cells in Mice

Creator:

Shan, X., Chen, L., Cao, M., Xu, L., and Zhang, S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9d2d8670-6774-47f9-935d-0e3f8969a417
uuid:9d2d8670-6774-47f9-935d-0e3f8969a417

Subject:

hsBAFF, B lymphocyte, CD4+ T lymphocyte, CD8+ T lymphocyte, and NK cell

Type:

article, model:article, and TEXT

Description:

B cell-activating factor belonging to the TNF family (BAFF, also called BLyS, TALL-1, zTNF-4, or THANK) is an important survival factor for B lymphocytes. In this study, we injected mouse abdominal cavity with human soluble BAFF (hsBAFF, 0.01, 0.1, 0.5, 2 mg/kg body mass) synthesized in Escherichia coli. On the 8th day after injection, we investigated the effects of hsBAFF on immune functional activities of splenic B lymphocytes, CD4+ and CD8+ T lymphocytes and natural killer (NK) cells in mice. The results showed that B lymphocyte proliferation significantly increased in hsBAFF-treated groups with dosages of 0.1 mg/kg (p<0.05), 0.5 and 2 mg/kg (p<0.01). We observed a dose-dependent increase of CD4+ T lymphocyte percentage and significantly higher values in 0.5 and 2 mg/kg hsBAFF-treated groups (p<0.05 and p<0.001, respectively) compared to control group, but CD8+ T lymphocyte percentage remained unchanged. The ratio of CD4+ to CD8+ T lymphocytes rose with increasing hsBAFF dosage (p<0.05 for 2 mg/kg hsBAFF vs. control). Significantly stronger NK cell activities were found in 0.5 and 2 mg/kg hsBAFF-treated groups (p<0.05). The main finding of this study is that the hsBAFF can enhance immune responses in the body by increasing B lymphocyte and CD4+ T lymphocyte function as well as elevating NK cell activity.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2006 Volume:55 | Number:3

Harvested from:

CDK

Metadata only:

false