A 33-35 kDa circulating antigen from Plasmodium falciparum

Title:

A 33-35 kDa circulating antigen from Plasmodium falciparum

Creator:

Dominguez, de Neira and Rodriguez-Acosta, Alexis

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9dc005e7-0cf2-4f1b-9a02-9a061572f127
uuid:9dc005e7-0cf2-4f1b-9a02-9a061572f127
urnnbn:urn:nbn:cz:aba007-000dts

Subject:

Plasmodium falciparum, soluble antigens, malaria, excretion antigens, hyperimmune serum, and thermal stability

Type:

model:internalpart and TEXT

Format:

print

Description:

Isolation and characterisation of Plasmodium falciparum (Welch, 1897) soluble antigens from infected patient plasma, Western blotting, thermal stability and ELISA assays using hyperimmune IgG-antimalaria antibodies was the main objective of this work. A circulating antigen of approximately Mr 33-35 kDa with good specificity and antigenicity, in the plasma of malarial patients was shown. Heating at 100°C did not destroy its antigenicity. When fractions highly enriched in the 33-35 kDa proteins were used in ELISAs, a seroreactivity in plasma obtained from primary-infected individuals was found. Controls from normal patients were always negative. The antigenic characteristics suggest that it may be included within the group of new described Plasmodium soluble antigens.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

267-272 and 29-34

Source:

Folia parasitologica | 2000 Volume:47 | Number:4

Harvested from:

CDK

Metadata only:

false