The effect of C-type nautriuretic peptide on delayed rectifier potassium currents in gastric antral circular myocytes of the guinea-pig

Title:

The effect of C-type nautriuretic peptide on delayed rectifier potassium currents in gastric antral circular myocytes of the guinea-pig

Creator:

Xu, H. Y., Huang, X., Yang, M., Sun, J.-B., Piao, L.-H., Zhang, Y., Gao, L., and Xu, Wen Xie

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a083741c-294a-42d1-98a2-5a1685446235
uuid:a083741c-294a-42d1-98a2-5a1685446235
issn:0862-8408

Subject:

Obecná zoologie, fyziologie živočichů, natriuretické peptidy, peptid, animal physiology, natriuretic peptides, stomach, delayed rectifier potassium currents, C-type natriuretic peptide, gastric myocytes, cyclic GMP, 2, and 591

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

C-type natriuretic peptides (CNP) play an inhibitory role in smooth muscle motility of the gastrointestinal tract, but the effect of CNP on delayed rectifier potassium currents is still unclear. This study was designed to investigate the effect of CNP on delayed rectifier potassium currents and its mechanism by using conventional whole-cell patch- clamp technique in guinea-pig gastric myocytes isolated by collagenase. CNP significantly inhibited delayed rectifier potassium currents [IK (V)] in dose-dependent manner, and CNP inhibited the peak current elicited by depolarized step pulse to 86.1±1.6 % (n=7, P<0.05), 78.4±2.6 % (n=10, P< 0.01) and 67.7±2.3 % (n=14, P<0.01), at concentrations of 0.01 μmol/l, 0.1 μmol/l and 1 μmol/l, respectively, at +60 mV. When the cells were preincubated with 0.1 μmol/l LY83583, a guanylate cyclase inhibitor, the 1 μmol/l CNP-induced inhibition of IK (V) was significantly impaired but when the cells were preincubated with 0.1 μmol/l zaprinast, a cGMP-sensitive phosphodiesterase inhibitor, the 0.01 μmol/l CNP-induced inhibition of IK (V) was significantly potentiated. 8-Br-cGMP, a membrane permeable cGMP analogue mimicked inhibitory effect of CNP on IK (V). CNP-induced inhibition of IK (V) was completely blocked by KT5823, an inhibitor of cGMP-dependent protein kinase (PKG). The results suggest that CNP inhibites the delayed rectifier potassium currents via cGMP-PKG signal pathway in the gastric antral circular myocytes of the guinea-pig., H. Y. Xu, X. Huang, M. Yang, J.-B. Sun, L.-H. Piao, Y. Zhang, L. Gao, W.-X. Xu., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:1

Harvested from:

CDK

Metadata only:

false