Effect of lithium on smooth muscle contraction and phosphorylation of myosin light chain by MLCK

Title:

Effect of lithium on smooth muscle contraction and phosphorylation of myosin light chain by MLCK

Creator:

Tang, Z. Y., Liu, Z. N., Chen, D. P., Fu, L., Ai, Q. D., and Lin, Y.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a3616b9b-d7dc-47b4-b270-513faec34e41
uuid:a3616b9b-d7dc-47b4-b270-513faec34e41
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, lithium, svaly, muscles, Phosphorylation of myosin light chain (MLC 20), Myosin Mg2+-ATPase, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The aims of our study were to investigate into the effect of lithium on smooth muscle contraction and phosphorylation of myosin light chain (MLC20) by MLCK and to find out the clue of its mechanism. Isolated rabbit duodenum smooth muscle strips were used to study the effects of lithium on their contractile activity under the condition of Krebs’ solution by means of HW-400S constant temperature smooth muscle trough. Myosin and MLCK were purified from the chicken gizzard smooth muscle. Myosin phosphorylation was determined by Glycerol-PAGE, myosin Mg2+-ATPase activity was measured by Pi liberation method. Lithium (10-40 mM) inhibited the contraction in duodenum in a dose-related and time-dependent manner. Lithium could also inhibit the extent of myosin phosphorylation in a dose-related and time-dependent manner, whereas it inhibited Mg2+-ATPase activity in a dose-rel ated manner. Lithium inhibited smooth muscle contraction by inhibition of myosin phosphorylation and Mg2+-ATPase activity., Z. Y. Tang, Z. N. Liu, L. Fu, D. P. Chen, Q. D. Ai, Y. Lin., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:6

Harvested from:

CDK

Metadata only:

false