Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae)

Title:

Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae)

Creator:

Tungjitwitayakul, Jatuporn, Tatun, Nujira, Vajarasathira, Boongeua, and Sakurai, Sho

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a4b9e611-1c1f-4810-b51a-e20514972737
uuid:a4b9e611-1c1f-4810-b51a-e20514972737
issn:1210-5759
doi:10.14411/eje.2016.017

Subject:

Zoologie, brouci, nosatcovití, beetles, Curculionid beetles, Curculionidae, Coleoptera, Sitophilus zeamais, UV-C, microwave, heat shock proteins, Hsp70, Hsc70, Hsp90, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, počítač, and online zdroj

Description:

The maize weevil, Sitophilus zeamais (Motschulsky), is a major pest of stored grain kernels. Irradiation is an established technique for controlling insects in stored grain and is a major stress factor affecting these insects. Since heat shock protein (hsp) genes respond to this stress, we proposed that hsps may be associated with irradiated stress tolerance in S. zeamais. The responses of the maize weevil to exposure to ultraviolet-C (UV-C) and microwave irradiation were assessed at four developmental stages: egg, larva, pupa and adult. The results revealed that exposure to UV-C (254 nm, < 8 h) did not affect the survival of the maize weevils; however, Szhsp70, Szhsc70 and Szhsp90 mRNA levels significantly increased during the first 1 h of UV-C exposure. The median lethal time (LT50) of exposure to microwave irradiation indicated that the adult stage was more tolerant of microwave irradiation than the other developmental stages. Microwave irradiation enhanced the expression of the three hsps, but the intensity of up-regulation differed among the three genes, with Szhsp70 the most highly up-regulated. Our experiments revealed that UV-C and microwave irradiation influenced the expression profile of hsp genes in S. zeamais. At the tissue level, the gene responses to UV-C and microwave irradiation varied greatly in different tissues., Jatuporn Tungjitwitayakul, Nujira Tatun, Boongeua Vajarasathira, Sho Sakurai., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

European Journal of Entomology | 2016 Volume:113

Harvested from:

CDK

Metadata only:

false