The flight activity of bats was studied at 21 localities in the Moravian Karst (Czech Republic). From April to October, bat detectors were used to record echolocation calls of bats on line transects during the first half of the night. Nine habitats were distinguished. In total, 666 minutes of the presence of flying bats and at least 16 bat species were registered during 3387 transect minutes. Myotis daubentonii was the most numerous species (46.2%) The number of bat species was the highest in rocky habitats (13 species), and the lowest in agrocoenoses (3 species). The greatest intensity of flight activity of the bat community was observed over ponds (35.0 min+/h) and streams (26.6 min+/h). With respect to habitat preference, M. mystacinus/brandtii, M. myotis/blythii, Eptesicus serotinus, Nyctalus noctula, Pipistrellus pipistrellus, and Plecotus auritus/austriacus appear to be eurytopic and M. daubentonii, M. nattereri and M. emarginatus to be stenotopic species.