Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains

Title:

Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains

Creator:

Jiřina Kolínská, Zákostelecká, M, Dvořák, B, Hana Kozáková, Goliáš, J, Lisa, V, and Zdeňka Zemanová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a5d263b9-d3b7-423a-83a1-9202db8d96ac
uuid:a5d263b9-d3b7-423a-83a1-9202db8d96ac
issn:0862-8408

Subject:

fyziologie člověka, human physiology, IEC-18, Lactobacillus, IL-18, TLRs, Caspase-1, Villin, β-catenin, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

Summary The aim of this study was to characterize an in vitro modulating effect of three commensal Lactobacillus strains on cellular differentiation of non-transformed crypt-like rat small intestinal cell line IEC-18. IEC-18 was grown on extracellular matrix, with or without presence of Lactobacillus strains. Gene expression of IEC-18 bacterial detection system - such as Toll-like receptors TLR-2, TLR-4, signal adapter MyD88, cytoplasmic NOD2 receptor, inflammatory cytokines IL-18, IL-1β, chemokine IL-8 and enzyme caspase-1 - was evaluated using real-time PCR. Expression and localization of TLR-2, TLR-4, IL-18 and caspase-1 proteins was demonstrated by Western blotting and immunofluorescent staining. Secretion of IL-18 to apical and basolateral surfaces was assayed by ELISA. Our results suggested that L. casei LOCK0919 accelerated differentiation of IEC-18 by stimulating TLR-2, TLR-4, MyD88, IL-18, caspase-1 mRNAs and proteins. L. casei LOCK0919 increased expression and transfer of villin and β-catenin from cytoplasm to cell membrane. Presence of L. rhamnosus LOCK0900 resulted in detachment of IEC-18 layer from extracellular matrix leading to induction of IL-1β, of TLR-2 and IL-8 mRNAs and stimulation of MyD88, caspase-1 and cytosolic receptor NOD2 mRNAs. L. rhamnosus LOCK0908 was not recognized by TLR-2 or TLR-4 receptors. Lactobacilli-IEC-18 crosstalk enhanced immune and barrier mucosal functions., J. Kolínská, M. Zákostelecká, Z. Zemanová, V. Lisa, J. Goliáš, H. Kozáková, B. Dvořák., and Seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:2

Harvested from:

CDK

Metadata only:

false