Glucose as a lipolytic agent: studies on isolated rat adipocytes

Title:

Glucose as a lipolytic agent: studies on isolated rat adipocytes

Creator:

Szkudelski, T. and Szkudelska, K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a758a6cf-fccd-45b7-a850-f2b24c0cb78e
uuid:a758a6cf-fccd-45b7-a850-f2b24c0cb78e
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, glukóza, glucose, adipocytes, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

In order to elucidate the direct effect of glucose on lipolysis in isolated rat adipocytes, cells were incubated in a buffer with different concentrations of this sugar: 2, 8 or 16 mmol/l. The increase in glucose concentration from 2 mmol/l to 8 or 16 mmol/l enhanced basal lipolysis by 30% and 47 %, respectively. Epinephrine-induced lipolysis (1 m mol/l) was also increased by 31 % and 32 %, when glucose concentration was increased from 2 mmol/l to 8 or 16 mmol/l, respectively. The rise in lipolysis caused by glucose was restricted by H-89 (an inhibitor of protein kinase A, 30 µmol/l), but insulin (1 nmol/l) had no inhibitory action. The augmentation of lipolysis by glucose did not require its metabolism (as demonstrated using 2-deoxyglucose) and was due to the action of this sugar on the final steps of the lipolytic cascade, particularly on protein kinase A. However, short-term exposure of adipocytes to higher glucose concentrations did not restrict the inhibitory action of insulin on lipolysis induced by epinephrine., T. Szkudelski, K. Szkudelska., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2000 Volume:49 | Number:2

Harvested from:

CDK

Metadata only:

false