New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

Title:

New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss

Creator:

Dyková, Iva, Kostka, Martin, Wortberg, Falk, Nardy, Elisabeth, and Pecková, Hana

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a761c204-524c-4cfd-b30c-fd3c4ab49bc8
uuid:a761c204-524c-4cfd-b30c-fd3c4ab49bc8
doi:10.14411/fp.2010.021

Subject:

nodular gill disease, aetiological study, amoebae, Naegleria sp., fish diseases, aquaculture, and Oncorhynchus mykiss

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus mykiss (Walbaum) in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (1), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperplastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

157-163

Source:

Folia parasitologica | 2010 Volume:57 | Number:3

Harvested from:

CDK

Metadata only:

false