Expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in HL-60 cells: dependence on cell cycle phases

Title:

Expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in HL-60 cells: dependence on cell cycle phases

Creator:

Michal Hofer, Ladislav Dušek, Zuzana Hoferová, Lenka Stixová, and Milan Pospíšil

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:abe60822-e3ca-4b43-8072-f5d66d3368d2
uuid:abe60822-e3ca-4b43-8072-f5d66d3368d2

Subject:

Fyziologie člověka a srovnávací fyziologie, adenosinové receptory, buněčný cyklus, adenosine receptors, cell cycle, messenger RNA expression, HL-60 promyelocytic cells, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

The present studies investigated changes in expression of mRNA for adenosine A1, A2a, A2b, and A3 receptors in samples of HL-60 promyelocytic cells differing in the actual presence of cells in various phases of the cell cycle induced by the double thymidine block method. Real-time PCR technique was used for obtaining data on mRNA expression. Statistical analysis of the data revealed that the mRNA ex pression of adenosine A1, A2a, and A3 receptors is dependent on the cell cycle phase. G0/G1 and G2/M phases were characterized by a higher mRNA expression of adenosine A1 receptors and a lower one of adenosine A2a and A3 receptors whereas the opposite was true for the S phase. Interestingly, expression of mRNA of the adenosine A2b receptors was independent on the cell cycle phase. The results indicate the plasticity of mRNA expression of adenosine receptors in the investigated promyelocytic cells and its interaction with physiological mechanisms of the cell cycle., M. Hofer ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:6

Harvested from:

CDK

Metadata only:

false