Orexins activates protein kinase C-mediated Ca2+ signaling in isolated rat primary sensory neurons

Title:

Orexins activates protein kinase C-mediated Ca2+ signaling in isolated rat primary sensory neurons

Creator:

Ozcan, M., Ahmet Ayar, Serhatlioglu, I., Alcin, E., Sahin, Z., and Kelestimur, H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:abe89e06-1d81-491b-a810-c66374973c1c
uuid:abe89e06-1d81-491b-a810-c66374973c1c

Subject:

Fyziologie člověka a srovnávací fyziologie, proteinové kinázy, protein kinases, orexin A, protein kinase C, calcium signaling, nociception, sensory neuron, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Previous results have suggested that orexins causes a rise of intracellular free calcium ([Ca2+]i) in cultured rat dorsal root ganglion (DRG) neurons, implicating a role in nociception, but the underlying mechanism is unknown. Hence, the aim of the present study was to investigate whether the orexins-mediated signaling involves the PKC pathways in these sensory neurons. Cultured DRG neurons were loaded with 1 μmol Fura-2 AM and [Ca2+]i responses were quantified by the changes in 340/380 ratio using fluorescence imaging system. The orexin-1 receptor antagonist SB-334867-A (1 μM) inhibited the calcium responses to orexin-A and orexin-B (59.1±5.1 % vs. 200 nM orexin-A, n=8, and 67±3.8 % vs. 200 nM orexin-B, n=12, respectively). The PKC inhibitor chelerythrine (10 and 100 μM) significantly decreased the orexin-A (200 nM)-induced [Ca2+]i increase (59.4±4.8 % P<0.01, n=10 and 4.9±1.6 %, P<0.01, n=9) versus response to orexin-A). It was also found that chelerythrine dose-dependently inhibited the [Ca2+]i response to 200 nM orexin-B. In conclusion, our results suggest that orexins activate intracellular calcium signaling in cultured rat sensory neurons through PKC-dependent pathway, which may have important implications for nociceptive modulation and pain., M. Ozcan ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:2

Harvested from:

CDK

Metadata only:

false