Comparison of the protein profiles of salivary gland extracts derived from three species of unfed and partially fed ixodid ticks analysed by SDS-PAGE

Title:

Comparison of the protein profiles of salivary gland extracts derived from three species of unfed and partially fed ixodid ticks analysed by SDS-PAGE

Creator:

Slovák, Mirko, Hajnická, Valéria, Labuda, Milan, and Fuchsberger, Norbert

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:acd996c6-a7a9-48ce-a743-7fcf62f591a7
uuid:acd996c6-a7a9-48ce-a743-7fcf62f591a7
urnnbn:urn:nbn:cz:aba007-000dt0

Subject:

tick saliva, SDS-PAGE, Ixodes ricinus, Haemaphysalis inermis, and Dermacentor reticulatus

Type:

model:internalpart and TEXT

Format:

print

Description:

Salivary gland extracts (SGE) from unfed and 5 days led adult female Ixodes ricinus (Linnaeus, 1758); Haemaphysalis inermis (Birula, 1895) and Dermacentor reticulatus (Fabricius, 1794) ticks were prepared. The protein content after feeding increased by 10.6, 8.7 and 6.8 limes, respectively. Extracts were equilibrated to the same protein content and submitted to SDS-polyacrylamide gel electrophoresis followed by computer analysis of the scanned gels. Relative differences in protein profiles of extracts obtained from unfed and partially fed ticks were found in all species and some of them were similar in all three species used in the study. Results demonstrate that the increase of the protein content in salivary glands during the feeding does not occur proportionally. Some proteins are synthesised preferentially (67.1 kDa, 13.5 kDa) but other bands (in range of 15-16 kDa) present in the SGE derived from unfed ticks arc less discernible in that of fed ticks.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

67-71 and 69-73

Source:

Folia parasitologica | 2000 Volume:47 | Number:1

Harvested from:

CDK

Metadata only:

false