Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex

Title:

Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex

Creator:

Fišerová, Lenka, Černá, Kateřina, Horká, Helena, and Kopecký, Jan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ae186ec1-279f-418b-9fa0-e02b298b95bd
uuid:ae186ec1-279f-418b-9fa0-e02b298b95bd
doi:10.14411/fp.2008.020

Subject:

Lyme disease spirochete, Borrelia burgdorferi, Borrelia garinii, Borrelia afzelii, tick, and Ixodes ricinus

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus (L.) ticks and three of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with ''classical'' infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44-65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naïve mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

150-154

Source:

Folia parasitologica | 2008 Volume:55 | Number:2

Harvested from:

CDK

Metadata only:

false