Purification and Properties of Ornithine Carbamoyltransferase from Loggerhead Turtle Liver

Title:

Purification and Properties of Ornithine Carbamoyltransferase from Loggerhead Turtle Liver

Creator:

Bellocco, E., Di Salvo, C., Lagan, G., Leuzzi, U., Tellone, E., Kotyk, A., and Galtieri, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:aef2dfad-aa2c-4c61-abdc-c238fb42960b
uuid:aef2dfad-aa2c-4c61-abdc-c238fb42960b
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Loggerhead turtle, Caretta caretta, Ornithine carbamoyltransferase, Enzyme kinetics, Enzyme thermostability, 14, and 612

Type:

article, studie, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Ornithine carbamoyltransferase has been purified from the liver of the loggerhead turtle Caretta caretta by a single-step procedure using chromatography on an affinity column to which the transition-state analogue, d-N-(phosphonoacetyl)- L-ornithine (d-PALO), was covalently bound. The procedure employed yielded an enzyme which was purified 373-fold and was judged to be homogeneous by nondenaturing and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme showed a specific activity of 224. The molar mass of the C. caretta enzyme was approximately 112 kDa, the single band obtained by SDS-PAGE indicated a subunit molar mass of 39.5 kDa; hence, the enzyme is a trimer of identical subunits. It catalyzes an ordered sequential mechanism in which carbamoyl phosphate binds first, followed by L-ornithine. The Michaelis constants were 0.858 mM for L-ornithine and 0.22 mM for carbamoyl phosphate, the dissociation constant of the enzyme-carbamoyl phosphate complex was 0.50 mM., E. Bellocco, C. Di Salvo, G. Lagan, U. Leuzzi, E. Tellone, A. Kotyk, A. Galtieri., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2002 Volume:51 | Number:2

Harvested from:

CDK

Metadata only:

false