MiR-18a inhibits PI3K/AKT signaling pathway to regulate PDGF-BB-induced airway smooth muscle cell proliferation and phenotypic transformation

Title:

MiR-18a inhibits PI3K/AKT signaling pathway to regulate PDGF-BB-induced airway smooth muscle cell proliferation and phenotypic transformation

Creator:

Yang, Wei, Chen, Yunbin, Huang, Chao, Wang, Wenjian, Huang, Congfu, and Li, Yuanguang

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b07a558e-b70c-43b1-96e7-b04411992336
uuid:b07a558e-b70c-43b1-96e7-b04411992336
doi:10.33549/physiolres.934753

Subject:

miR-18a, PDGF-BB, airway smooth muscle cells (ASMCs), airway remodeling, and PI3K/AKT pathway

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

The increased proliferation and migration of airway smooth muscle cells (ASMCs) is a key process in the formation of airway remodeling in asthma. In this study, we focused on the expression of mircoRNA-18a (miR-18a) in airway remodeling in bronchial asthma and its related mechanisms. ASMCs are induced by platelet-derived growth factor BB (PDGF-BB) for in vitro airway remodeling. The expression of miR-18a in sputum of asthmatic patients and healthy volunteers was detected by qRT-PCR. The expression of miR-18a was over-expressed or interfered with in PDGF-BB-treated ASMCs. Cell proliferation, apoptosis and migration were detected by MTT, flow cytometry and Transwell, respectively, the expression of contractile phenotype marker proteins (SM-22α, α-SM-actin, calponin) and key molecules of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway (PI3K, p-PI3K, AKT and p-AKT) in ASMCs were detected by Western blot. The expression of miR-18a was down-regulated in the sputum and PDGF-BB-treated ASMCs of asthma patients. PDGF-BB could promote the proliferation and migration of ASMCs and inhibit their apoptosis, it could also promote the phenotypic transformation of ASMCs and activate the PI3K/AKT pathway. MiR-18a could inhibit the proliferation, migration ability and phenotypic transformation of ASMCs induced by PDGF-BB to a certain extent and alleviate the effect of PDGF-BB in supressing apoptosis, while miR-18a could inhibit the activation of the PI3K/AKT pathway. MiR-18a inhibits PDGF-BB-induced proliferation, migration and phenotypic conversion of ASMCs by inhibiting the PI3K/AKT pathway, thus attenuating airway remodeling in asthma.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

883-892

Source:

Physiological research | 2021 Volume:70 | Number:6

Harvested from:

CDK

Metadata only:

false