Microvascular endothelial cells from human omentum lack an inward rectifier K+ current

Title:

Microvascular endothelial cells from human omentum lack an inward rectifier K+ current

Creator:

Himmel, H. M., Rauen, U., and Ravens, U.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b1f0025e-a4fa-43d9-8271-b87352db31ab
uuid:b1f0025e-a4fa-43d9-8271-b87352db31ab
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, whole-cell voltage clamp, ion substitution, non-excitable cells, Na+ background current, chloride conductance, CA 2+ - activated K+ current, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

In most macrovascular endothelial cell (EC) preparations, resting membrane potential is determined by the inwardly rectifying K+ current (IK1), whereas in microvascular EC the presence of IK1 varies markedly. Cultured microvascular EC from small vessels of human omentum were examined by means of the voltage-clamp technique to elucidate the putative role of IK1 in maintaining resting membrane potential. Macrovascular EC from human iliac artery and bovine aorta served as reference. Human omentum EC showed an outwardly rectifying current-voltage relation. Inward current was hardly sensitive to variations of extracellular [K+] and Ba2+ block suggesting lack of IK1. However, substitution of extracellular [Na+] and/or [Cl-] affected the current-voltage relation indicating that Na+ and Cl- contribute to basal current. Furthermore, outward current was reduced by tetraethylammonium (10 mM), and cell-attached recordings suggested the presence of a Ca2+-activated K+ current. In contrast to human omentum EC, EC from human iliac artery and bovine aorta possessed inwardly rectifying currents which were sensitive to variations of extracellular [K+] and blocked by Ba2+. Thus, the lack of IK1 in human omentum EC suggests that resting membrane potential is determined by Na+ and Cl- currents in addition to K+ outward currents., H. M. Himmel, U. Rauen, U. Ravens., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2001 Volume:50 | Number:6

Harvested from:

CDK

Metadata only:

false