Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites

Title:

Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites

Creator:

Cámara, María de los Milagros, Bouvier, León A., Miranda, Mariana R., Reigada, Chantal, and Pereira, Claudio A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b32ef4b5-4145-475c-a402-f7bb80dc0248
uuid:b32ef4b5-4145-475c-a402-f7bb80dc0248
doi:10.14411/fp.2013.042

Subject:

Chagas disease, metacyclogenesis, parasite differentiation, and intracellular epimastigotes

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

406-410

Source:

Folia parasitologica | 2013 Volume:60 | Number:5

Harvested from:

CDK

Metadata only:

false