Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control

Title:

Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control

Creator:

Isasawin, Siriwan, Aketarawong, Nidchaya, and Thanaphum, Sujinda

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b40ea509-3a67-498c-932d-778e53ca63f1
uuid:b40ea509-3a67-498c-932d-778e53ca63f1

Subject:

Zoologie, zoologie, entomologie, Tephritidae, oriental fruit fly, Bactrocera dorsalis, microsatellites, genetic sexing strain, mating competitiveness, sterile insect technique, 2, and 59

Type:

article, články, model:article, and TEXT

Description:

The oriental fruit fly, Bactrocera dorsalis, is a key economic insect pest reducing fruit yield and generating constraints in the international market. The application of the sterile insect technique (SIT) continues to reveal areas where new technologies can improve the effectiveness of fruit fly control. One such advancement concerns insect strains. In the present study, a mass-reared strain of the fly with a translocation-based genetic sexing character (Salaya1) based on a brown-white pupal colour dimorphism was genetically characterized using 11 microsatellite DNA markers. Subsequently, these markers were used to evaluate the maintenance of genetic variability in the strain under mass-rearing conditions. Mating competitiveness of this strain was also tested in field cages. Two of the newly characterized Y-pseudo-linked microsatellite markers were used for strain identification in field monitoring traps. The strain was also validated in a pilot integrated pest management (IPM) programme using male-only SIT in a fruit orchard. The programme resulted in the suppression of the fruit fly population., Siriwan Isasawin, Nidchaya Aketarawong, Sujinda Thanaphum., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2012 Volume:109 | Number:3

Harvested from:

CDK

Metadata only:

false