After Neogobius kessleri, Neogobius gymnotrachelus, and Neogobius fluviatilis, Neogobius melanostomus is the fourth species of Gobiidae to find its way up the River Danube to Slovakia, occurring in our samples near Štúrovo (river km 1720) and in the River Hron (river km 0.82). The fish (male, females, juvenile) were captured along weakly sloped sandy riverbanks with large stones. The mensural and meristic characters of the specimens were similar to those reported elsewhere for the species.