Activities of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase/oxygenase in leaves and fruit pericarp tissue of different coffee (Coffea sp.) genotypes

Title:

Activities of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase/oxygenase in leaves and fruit pericarp tissue of different coffee (Coffea sp.) genotypes

Creator:

Lopez, Y., Riaño, N., Mosquera, P., Cadavid, A., and Arcila, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b7509087-c57b-4d91-a3f0-d5aa5c9954cb
uuid:b7509087-c57b-4d91-a3f0-d5aa5c9954cb
issn:0300-3604
doi:10.1023/A:1007265715108

Subject:

chclorophyll, enzyme activity, maize, Michaelis-Menten constants, total leaf proteins, and Zea mays

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

In order to study photosynthetic characteristics, phosphoenolpyruvate carboxylase (PEPC) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities as well as soluble protein and chlorophyll contents were determined in leaf and fruit pericarp samples from diverse coffee genotypes (Coffea arabica cv. Colombia, Caturra, Caturra Erecta, San Pacho, Tipica, C. stenophylla, C. eugenioides, C. congensis, C. canephora, C. canephora cv. Arabusta, C. arabica cv. Caturra×C. canephora and Hibrido de Timor. We found a slightly higher PEPC activity in fruit pericarp than in leaves, while RuBPCO activity was much lower in pericarp than leaf tissue. Partial purification of PEPC and RuBPCO was carried out from leaves of C. arabica cv. Caturra and Michaelis-Menten kinetics for RuBPCO (Km CO2 = 5.34 µM), (Km RuBP = 9.09 µM) and PEPC (Km PEP = 19.5 µM) were determined. Leaf tissues of Colombia, Hibrido de Timor, and Caturra consistently showed higher content of protein [55.4-64.4 g kg-1 (f.m.)] than San Pacho, C. stenophylla, Tipica, Caturra Erecta, and Caturra×C. canephora [25.6-36.9 g kg-1 (f.m.)] and C. canephora cv. Arabusta, Borbon, C. congensis, C. eugenioides, and C. canephora [16.1-21.1 g kg-1 (f.m.)]. and Y. Lopez ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

215-220

Source:

Photosynthetica | 2000 Volume:38 | Number:2

Harvested from:

CDK

Metadata only:

false