Detection of a single nucleotide polymorphism (SNP) DNA marker linked to cocoon traits in the mulberry silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Title:

Detection of a single nucleotide polymorphism (SNP) DNA marker linked to cocoon traits in the mulberry silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Creator:

Sreekumar, Sivaramakurup, Ashwath, Southekal K., Slathia, Monika, Kumar, Sundaramurthy N., and Qadri, Syed M. H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bbf9de04-6334-4f2c-8e30-7a4e0b88d929
uuid:bbf9de04-6334-4f2c-8e30-7a4e0b88d929

Subject:

Zoologie, entomologie, Bombyx mori, bulked segregant analysis, cocoon traits, single nucleotide polymorphism, quantitative trait loci, QTL, 2, and 59

Type:

article, články, model:article, and TEXT

Description:

Cocoon weight and shell weight are the key economic traits ultimately determining silk yield. In order to detect the main quantitative trait loci (QTL) associated with the cocoon traits of the mulberry silkworm, Bombyx mori, the parents of larvae that produced cocoons that differed greatly in weight and shell weight were screened using 240 primer pairs of single nucleotide polymorphic markers (SNPs) representing all the 28 linkage groups in silkworm. Out of the 240 primer pairs, 48 (20%) revealed distinct polymorphism between the parents, which was confirmed by the co-dominant expression of both polymorphic PCR products in the F1 generation. The bulked segregant analysis (BSA) was used to compare the SNP profiles of the parents, F1 and F2 bulks using the 48 informative SNP primers. This revealed that out of 48 primer pairs, only one pair, i.e., No. 04124 of the linkage group 4 showed clear differences in the amplified products between the bulks corresponding to that of the parents with different cocoon traits suggesting that the DNA regions amplified by this primer pair are closely linked to the QTL controlling the cocoon traits. The results were also confirmed by screening the backcross (BC) progeny. This is the first report of the identification of a QTL using SNPs with BSA. The results of the present study indicate that it might be possible to use SNPs for marker assisted selection (MAS) in silkworm breeding programs aimed at improving cocoon traits. and Sivaramakurup Sreekumar, Southekal K. Ashwath, Monika Slathia, Sundaramurthy N. Kumar, Syed M.H. Qadri.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2011 Volume:108 | Number:3

Harvested from:

CDK

Metadata only:

false