Vasorelaxing action of vasonatrin peptide is associated with activation of large-conductance Ca2+-activated potassium channels in vascular smooth muscle cells

Title:

Vasorelaxing action of vasonatrin peptide is associated with activation of large-conductance Ca2+-activated potassium channels in vascular smooth muscle cells

Creator:

Yu, J., Zhu, M., Fu, Z., Zhu, X., Zhao, Y., and Chen, B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bcd7c40c-4fc6-4f3e-b3f3-57d7b0c1def5
uuid:bcd7c40c-4fc6-4f3e-b3f3-57d7b0c1def5

Subject:

Fyziologie člověka a srovnávací fyziologie, natriuretické peptidy, natriuretic peptides, vasonatrin peptide, vascular smooth muscle cells, calcium-activated potassium channels, cyclic guanosine monophosphate, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

The aim of this study was to test the hypothesis that vasorelaxing action of vasonatrin peptide (VNP) is due to activation of the large-conductance Ca2+-activated potassium channel (BKCa) via guanylyl cyclase (GC)-coupled natriuretic peptide receptors (NPRs) in vascular smooth muscle cells (VSMCs). Contraction experiments were performed using human radial artery, whereas BKCa current by patch clamp was recorded in cells from rat mesenteric artery. Contractility of rings cut from human radial artery was detected in vitro. As a result, VNP induced a dose-dependent vasorelaxation of human radial artery, which could be mimicked by 8-Br-cGMP, and suppressed by TEA, a blocker of BKCa, HS-142-1, a blocker of GC-coupled NPRs, or methylene blue (MB), a selective inhibitor of guanylyl cyclase. Sequentially, whole-cell K+ currents were recorded using patch clamp techniques. BKCa current of VSMCs isolated from rat mesentery artery was obtained by subtracting the whole cell currents after applications of 10-7 mol/l iberiotoxin (IBX) from before its applications. In accordance with the results of arterial tension detection, BKCa current was significantly magnified by VNP, which could also be mimicked by 8-Br-cGMP, whereas suppressed by HS-142-1, or MB. Taken together, VNP acts as a potent vasodilator, and NPRA/B-cGMP-BKCa is one possible signaling system involved in VNP induced relaxation., J. Yu ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:2

Harvested from:

CDK

Metadata only:

false