Potentiation of GABAa receptor in cultured mouse hippocampal cells by brain-derived peptide mixture cerebrolysin

Title:

Potentiation of GABAa receptor in cultured mouse hippocampal cells by brain-derived peptide mixture cerebrolysin

Creator:

Zemková, H., Krůšek, J., and Vyskočil, F.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bcddb004-1ce1-4935-9ef6-78564bb65bd6
uuid:bcddb004-1ce1-4935-9ef6-78564bb65bd6

Subject:

mouse hippocampal neurones, cerebrolysin, GABA A receptor, NMDA receptor, non-NMDA receptor, and patch clamp

Type:

article, model:article, and TEXT

Description:

Application of Cerebrolysin (0.1 /rg per 1 ml) by a fast microperfusion system induced an inward current of 0.2 to 1 nA in all neurones from newborn mouse hippocampi held at —30 mV membrane potential. Cerebrolysin- induced currents were reduced by the GABAa antagonist bicuculline (2 /¿M) by 65 %, by the NMDA antagonist aminophosphovaleric acid (APV, 10 /¿M) by 27 %, and by the non-NMDA antagonist cyanonitroquinoxalinedione (CNQX, 10 ¡utA) by 20 %. Cerebrolysin dialyzed through a 3.6 kD gut did not induce any transmembrane current but potentiated the response induced by GABA (10 /utA) to 135 %. We conclude that, in addition to amino acids which activate GABAa, NMDA and non-NMDA receptors, Cerebrolysin also contains a peptide which potentiates the GABAa receptor response.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1995 Volume:44 | Number:3

Harvested from:

CDK

Metadata only:

false