Photothermal spectra of thylakoids isolated from cucumber cotyledons at various stages of greening

Title:

Photothermal spectra of thylakoids isolated from cucumber cotyledons at various stages of greening

Creator:

Waloszek, A., Więckowski, S., Planner, A., Boguta, A., and Frąckowiak, D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:be03b856-98e0-4314-b92c-8cd67300a6b8
uuid:be03b856-98e0-4314-b92c-8cd67300a6b8
issn:0300-3604
doi:10.1023/A:1021310111044

Subject:

Cucumis sativus, laser-induced optoacoustic spectra, and photoacoustic steady state spectra

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The character of interaction between carotenoids (Cars) and chlorophylls (Chls) in thylakoids isolated from cucumber cotyledons at three stages of greening (3, 6, and 24 h of irradiation with 120 µmol m-2 s-1) was studied. The shapes of the steady state photoacoustic spectra were changed with the change in time of greening and with the frequency of radiation modulation. The shapes show that changes not only in the contents of various pigments but also in pigment interactions with surrounding occur and that processes of thermal deactivation characterised by different kinetics take place. Slow processes of thermal deactivation are in most cases due to deactivation of triplet states. Long living triplet states are very often engaged in photochemical reactions that can destroy the tissue. Analysis of the time-resolved photothermal spectra shows that at later stage of greening, the chlorophyll (Chl) molecules are better shielded against photo-destruction because Cars more efficiently quench their triplet states. The yield of formation of the pigment triplet states measured by the time resolved photothermal method, always at the same energy absorbed by pigment mixture, declined during sample greening. The decay time of the slow component of pigment thermal deactivation, due predominantly to deactivation of the triplet state of Chl, decreases with the increase of time of greening from 6.2 µs for the 3-h sample to 1.5 µs for the 24 h sample. The energy taken by Cars from Chls is dissipated into heat, therefore the steady state and quick thermal deactivation values increased during the greening process. The Cars/Chls ratio in the thylakoids decreased during greening approximately 2 fold. Hence at a later phase of greening the Cars can quench the triplet states of Chls more efficiently than at an earlier phase of greening. and A. Waloszek ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

279-288

Source:

Photosynthetica | 2002 Volume:40 | Number:2

Harvested from:

CDK

Metadata only:

false